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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Methods Mol Biol. 2012;843:29–46. doi: 10.1007/978-1-61779-523-7_4

Fig. 3.

Fig. 3

An example of a splice-blocking MO targeting the X. laevis gene Castor (Cst). (a) The position of the Cst splice junction morpholinos relative to the pre-mRNA transcript. These MOs target the splice donor site on exon 8 (ex8D MO) and the splice acceptor site on exon 9 (ex9A MO). (b) RT-PCR analysis of stage 42 X. laevis tadpoles injected at the one-cell stage with both the ex8D and ex9A MOs, demonstrating the inhibition of proper splicing of Cst pre-mRNA and the inclusion of a 679-bp intron, as indicated by the larger amplified product compared to control (Con) MO and 5-mismatch (5-mis) MO. Abrogation of splicing in this context introduced a stop codon after the second amino acid following exon 8. Reprinted from Christine and Conlon (23), Copyright (2008), with permission from Elsevier.