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Isocitrate dehydrogenase activity in exponentially growing E. coli cultures. E. coli parental and SOD-deficient strains were grown in LB medium to the indicated densities. The cells were washed, disrupted by sonication and ICD activity was measured in 1.0 ml reaction mixture contained 33 mm Tris-EDTA buffer (pH 7.4), 1.33 mm MnCl2, 1.3 mm DL-Isocitrate and 0.1 mm NADP+. Experiments were repeated three times with 3–5 replicates. Bars represent means±SE.
