Table 1.
Annotation of the proteins in the grapevine apoplastic fluid leaf
| Classesa | Subclassesb | Spots n°c | Gi acc. n°d | Loc.e | Closer homologous protein by Blastp [species]f | % hom.g |
|---|---|---|---|---|---|---|
| PR proteins |
chitinases |
19;55;127;134;135 |
157329111 |
C |
chitinase, putative [Ricinus communis] |
75 |
| |
31 |
15213852 |
S |
chitinase-B [Sorghum halepense] |
100 |
|
| |
44 |
225462669 |
S |
class III chitinase [Vitis vinifera] |
99 |
|
| |
55;59;121;122;125;127;128;134;135 |
225469348 |
S |
chitinase, putative [Ricinus communis] |
77 |
|
| |
65 |
10880381 |
S |
chitinase [Vitis vinifera] |
100 |
|
| |
140;141;175 |
116333 |
S |
chitinase B [Zea mays] |
100 |
|
| |
142 |
147820457 |
S |
chitinase 1 precursor, putative [Ricinus communis] |
76 |
|
| |
144 |
266324 |
NC |
chitinase-B [Sorghum halepense] |
62 |
|
| |
147;148;149 |
225454408 |
S |
acidic chitinase III [Nicotiana tabacum] |
82 |
|
| |
155 |
225434076 |
C |
class IV chitinase [Vitis pseudoreticulata] |
87 |
|
| |
176 |
157353734 |
C |
class IV chitinase [Vitis pseudoreticulata] |
87 |
|
| glucanases |
136 |
163914215 |
S, TM |
beta 1–3 glucanase [Vitis hybrid cultivar] |
100 |
|
| |
39;40;45;46;47;63;136 |
225441375 |
S, TM |
beta 1–3 glucanase [Vitis vinifera] |
99 |
|
| |
24;41;42;61;64;136 |
225441379 |
S |
beta-1,3-glucanase [Vitis riparia] |
99 |
|
| |
27;167;168;169 |
225441373 |
S, TM |
beta-1,3-glucanase [Vitis riparia] |
86 |
|
| |
22 |
170243 |
S, TM |
beta-1,3-glucanase [Vitis vinifera] |
84 |
|
| other PR proteins |
35 |
225429115 |
S |
pathogenesis-related protein 1 [Vitis hybrid cultivar] |
100 |
|
| |
36 |
225429250 |
S |
pathogenesis-related protein 1 [Vitis hybrid cultivar] |
88 |
|
| |
37 |
225429119 |
S |
putative pathogenesis-related protein 1 [Vitis hybrid cultivar] |
98 |
|
| |
33;34;36;49;160;161;163;173 |
225426801 |
S, TM |
osmotin-like protein [Vitis vinifera] |
90 |
|
| |
177 |
7406714 |
M |
putative thaumatin-like protein [Vitis vinifera] |
100 |
|
| |
162;177 |
225426793 |
S, TM |
thaumatin-like protein [Vitis vinifera] |
99 |
|
| |
145;146;150;151;156 |
225444754 |
S |
germin-like protein 6 [Vitis vinifera] |
100 |
|
| |
152;153;154;157;159 |
225429295 |
S |
NtPRp27 [Nicotiana tabacum] |
83 |
|
| |
166 |
147853970 |
UC |
pathogenesis-related protein 10.7 [Vitis vinifera] |
73 |
|
| |
164 |
225453020 |
S |
Wound-induced protein WIN2 precursor, putative [Ricinus communis] |
82 |
|
| proteases |
serine proteinases |
83 |
225449346 |
NC |
cucumisin precursor, putative [Ricinus communis] |
76 |
| |
3;80;81;82;83;89;90;94;95;119 |
157344189 |
NC |
cucumisin precursor, putative [Ricinus communis] |
78 |
|
| |
68;70;71;72 |
157335112 |
NC |
subtilisin-type protease precursor [Glycine max] |
80 |
|
| |
69 |
1771160 |
S |
xylem serine proteinase 1 precursor, putative [Ricinus communis] |
85 |
|
| |
110 |
157335622 |
NC |
xylem serine proteinase 1 precursor, putative [Ricinus communis] |
85 |
|
| |
4;5 |
157345245 |
S, TM |
xylem serine proteinase 1 precursor, putative [Ricinus communis] |
89 |
|
| |
111 |
225457767 |
S |
serine carboxypeptidase, putative [Ricinus communis] |
88 |
|
| |
172 |
157348245 |
S, TM |
serine carboxypeptidase, putative [Ricinus communis] |
79 |
|
| aspartic proteinases |
48;115;117;118;119;123;126 |
157335788 |
M |
aspartic proteinase nepenthesin-1 precursor, putative [Ricinus communis] |
85 |
|
| |
14;18;129 |
157339844 |
S |
aspartic proteinase nepenthesin-2 precursor, putative [Ricinus communis] |
75 |
|
| |
15;16;17;57;99;102;103;120;124 |
225430555 |
S |
aspartic proteinase nepenthesin-2 precursor, putative [Ricinus communis] |
76 |
|
| cell wall metabolism |
hydrolases |
28;29;171 |
147771556 |
S, TM |
xyloglucan endotransglucosylase/hydrolase [Gossypium hirsutum] |
90 |
| |
67 |
157354845 |
S, TM |
glycosyl hydrolase family 38 protein [Arabidopsis thaliana] |
77 |
|
| glucosidases |
1 |
147821903 |
S, TM |
alpha-glucosidase, putative [Ricinus communis] |
87 |
|
| |
66 |
147787240 |
S |
alpha-glucosidase, putative [Ricinus communis] |
79 |
|
| |
2;77;78;79;107;108;109 |
225423961 |
S, TM |
alpha-glucosidase, putative [Ricinus communis] |
90 |
|
| |
84;85 |
157350003 |
S |
beta-glucosidase, putative [Ricinus communis] |
90 |
|
| |
84;85;86;87;88;96 |
157355824 |
S, TM |
beta-glucosidase, putative [Ricinus communis] |
90 |
|
| galactosidases |
101 |
147810287 |
UC |
alpha-galactosidase/alpha-n-acetylgalactosaminidase, putative [Ricinus communis] |
92 |
|
| |
58 |
157329180 |
NC |
beta-galactosidase, putative [Ricinus communis] |
87 |
|
| |
13;50;53;56;98;174 |
157337481 |
S, TM |
beta-galactosidase, putative [Ricinus communis] |
89 |
|
| |
97;112 |
157332401 |
S |
beta-galactosidase, putative [Ricinus communis] |
88 |
|
| fucosidase |
15;53;54 |
225424647 |
S, TM |
alpha-L-fucosidase 2 precursor, putative [Ricinus communis] |
82 |
|
| arabinofuranosidase |
3;6;7 |
225440254 |
S, TM |
alpha-N-arabinofuranosidase 1 precursor, putative [Ricinus communis] |
83 |
|
| polygalacturonase |
81 |
225426168 |
S, TM |
polygalacturonase, putative [Ricinus communis] |
88 |
|
| pectinesterases |
43;49 |
157353359 |
NC |
pectinesterase-2 precursor, putative [Ricinus communis] |
71 |
|
| |
56;130 |
157350105 |
S, TM |
pectinesterase-3 precursor, putative [Ricinus communis] |
78 |
|
| |
73 |
157353908 |
S |
pectin methylesterase 2 [Pyrus communis] |
86 |
|
| peroxidases |
|
23;26;29;62 |
225459180 |
S |
cationic peroxidase [Arachis hypogaea] |
85 |
| |
60 |
157355449 |
S, TM |
class III peroxidase [Gossypium hirsutum] |
86 |
|
| |
21;25 |
157355447 |
S, TM |
class III peroxidase [Gossypium hirsutum] |
89 |
|
| |
20;75;76;105;106 |
225435616 |
S |
peroxidase [Armoracia rusticana] |
80 |
|
| |
131;158 |
223635592 |
NC |
peroxidase 1 [Scutellaria baicalensis] |
80 |
|
| |
131 |
157342951 |
S |
peroxidase 25 precursor, putative [Ricinus communis] |
83 |
|
| other stress related proteins |
|
165 |
134684 |
C |
putative Cu/Zn superoxide dismutase precursor [Vitis vinifera] |
83 |
| |
127 |
26224736 |
NC |
serpin-like protein [Citrus x paradisi] |
85 |
|
| |
113;114 |
147846526 |
S |
reticuline oxidase precursor, putative [Ricinus communis] |
80 |
|
| |
132;133 |
147825300 |
S, TM |
reticuline oxidase precursor, putative [Ricinus communis] |
82 |
|
| |
21 |
76559894 |
UC |
isoflavone reductase-like protein 5 [Vitis vinifera] |
100 |
|
| |
10;11;12 |
157360089 |
S |
heparanase, putative [Ricinus communis] |
84 |
|
| |
43 |
115488670 |
C |
universal stress protein family protein, expressed [Oryza sativa Japonica Group] |
100 |
|
| micellaneous |
|
138;139;170 |
225443264 |
S |
acid phosphatase [Glycine max] |
79 |
| |
104 |
147814943 |
S, TM |
alpha-amylase [Vigna angularis] |
81 |
|
| |
90 |
91983977 |
UC |
ATP synthase CF1 alpha subunit [Vitis vinifera] |
100 |
|
| |
81 |
224365667 |
UC |
ATPase alpha subunit [Vitis vinifera] |
100 |
|
| |
137 |
157343592 |
UC |
chloroplast heat shock protein 70–2 [Ipomoea nil] |
93 |
|
| |
52 |
225466690 |
C |
chloroplast sedoheptulose-1,7-bisphosphatase [Solanum lycopersicum] |
95 |
|
| |
32 |
225457957 |
UC |
cyclophilin [Catharanthus roseus] |
94 |
|
| |
124 |
225454430 |
S, TM |
lanatoside 15′-O-acetylesterase [Digitalis lanata] |
84 |
|
| |
83 |
157343878 |
S |
lysosomal alpha-mannosidase, putative [Ricinus communis] |
86 |
|
| |
20;92;93 |
157343877 |
UC |
lysosomal alpha-mannosidase, putative [Ricinus communis] |
83 |
|
| |
51;52;74 |
157341194 |
S |
lysosomal alpha-mannosidase, putative [Ricinus communis] |
87 |
|
| |
143 |
225440390 |
NC |
NAD-dependent epimerase/dehydratase [Zea mays] |
92 |
|
| |
32;38 |
225453348 |
UC |
nucleoside diphosphate kinase B [Jatropha curcas] |
95 |
|
| |
119 |
147769722 |
C |
polyprotein [Oryza australiensis] |
58 |
|
| |
100 |
11385598 |
M |
putative chloroplast RNA helicase VDL’ isoform 2 [Nicotiana tabacum] |
100 |
|
| |
8 |
149774708 |
NC |
ribulose-1,5-bisphosphate carboxylase/oxygenase [Ophioglossum petiolatum] |
100 |
|
| |
9 |
147769051 |
UC |
ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit [Vitis vinifera] |
94 |
|
| |
116 |
225436591 |
UC |
serine-pyruvate aminotransferase, putative [Ricinus communis] |
95 |
|
| |
19 |
116830469 |
C |
hypothetical protein [Arabidopsis thaliana] |
100 |
|
| |
30 |
125563554 |
UC |
hypothetical protein [Vitis vinifera] |
49 |
|
| |
69 |
157343556 |
C |
hypothetical protein [Vitis vinifera] |
100 |
|
| 91 | 147818959 | NC | Ca/calmodulin-dependent protein kinase | 40 |
a the protein distribution according to their biological process.
b the protein group.
c the number that identifies protein spots on 2-D apoplastic map.
d the accession number from GenBank assigned to the peptide after MS/MS analysis.
e the predicted localisation of the peptide according to the presence of a N-terminal signal peptide (S), non-classical signal peptide (NC), transmembrane helices (TM), chloroplast (C), mitochondria (M) and unclassified (UC) location.
f the peptide identification based on homology to proteins characterized in other species by Blast-p search on NCBI database.
g the relative homology percentage of protein obtained by Blast-p.
Shared proteins identified in both AF sample and total leaf sample are highlighted in bold.