Table 3.
Purity of extracts in routine casework using Microsep 30 K and Amicon Ultra 30 K
Sample type | Inhibition following Microsep 30 K purification (1 May 2011 to 31 August 2011) | Inhibition following Amicon Ultra 30 K purification (1 October 2011 to 29 February 2012) |
---|---|---|
Rape case samples |
41% (n = 1,432) |
16% (n = 2,094) |
Hairs |
31% (n = 94) |
26% (n = 130) |
Touch stains on mini-tape |
43% (n = 1,439) |
26% (n = 2,641) |
Organically extracted samples | 52% (n = 21) | 11% (n = 18) |
Crime scene DNA extracts are defined as PCR-inhibitory if they generate a shift of the Quantifiler Human IPC Cq compared to the normal value for pure reactions.