Table 3.
Genes that showed a significant difference in their expression between wild-type and mef2A-mutant structures developed for 16 hours, as determined by mRNA sequencing
| Increased expression in the mutant | Decreased expression in the mutant | |
|---|---|---|
| hssA-related genes (prestalk-specific) |
G0267936 -93 (93–1) |
G0293362 10.74 (26–279) |
| G0268400 -80 (160–2) |
G0281013 11.95 (19–227) |
|
| G0277741 -55.6 (1724–31) |
|
|
| G0281001 -31.2 (780–25) | ||
| G0281189 -134.75 (539–4) | ||
| G0281191 0 (79–0) | ||
| G0281195 -35.33 (106–3) | ||
| G0281197 -55.5 (111–2) | ||
| G0282307 -106.67 (320–3) | ||
| G0283713 -72.75 (291–4) | ||
| G0293356 -11.24 (281–25) | ||
| hssA. -10 (1550–155) | ||
| Small proteins (57–59 aa) (prestalk specific) |
G0283421 -34.33 (515–15) |
|
| G0283465 -118.67 (356–3) | ||
| G0283501 -25.19 (3929–156) | ||
| G0283503 -41.42 (994–24) | ||
| G0283505 -33.95 (1935–57) | ||
| G0283507 -38.25 (1224–32) | ||
| G0283511 -9.55 (2015–211) | ||
| G0283515 -14.28 (2313–162) | ||
| G0283519 -55.88 (950–17) | ||
| G0272188 -15.28 (2119–139) | ||
| G0269674 -15.57 (794–51) | ||
| G0284283 (111–0) | ||
| G0283395 (97–0) | ||
| G0271888 -40.5 (81–2) | ||
| G0269672 -7.46 (574–77) | ||
| Small proteins (69–72 aa) (prespore specific) |
|
G0285863 28.07 (208–7523) |
| G0284623*c 35.48 (29–1029) | ||
| G0271110* 13.91 (11–153) | ||
| Tiger family proteins |
tgrF1. -39.85 (518–13) |
tgrC5*c 18.9 (10–189) |
| Polyketide synthase family |
Pks32 -6.62 (1205–182) |
|
| Transcription regulation |
mybC −10.86 (228–21) |
comH*ca 11.53 (184–2123) |
| G0288967* 17.07 (96–1639) | ||
| G0290847*a 21.74 (80–1739) | ||
| G0290855*c 32.39 (41–1328) | ||
| G0271438* 65.5 (4–262) | ||
|
srfC. 0 (0–73) | ||
| Developmental genes |
St15 -7.69 (1084–171) |
psiI* 26.06 (469–12220) |
| psiN −41.51 (5645–136) | ||
| Signaling proteins |
Omt12 -19.79 (277–14) |
hspC*ca 15.69 (26–408) |
| arrK −10.65 (213–20) | ||
| Metabolism |
osbH −38.33 (115–3) |
fhbB 6.54 (525–3433) |
| G0278647 -23.43 (164–7) | ||
| Putative Membrane proteins |
G0275535 -6.03 (3197–530) |
G0285697* 451.2 (5–2256) |
| G0289143 -9.79 (1116–114) |
G0267564* 16.32 (41–669) |
|
| G0287195 -10.05 (774–77) |
G0272714* 10.99 (73–802) |
|
| G0284683 -11.87 (273–23) |
G0270342* 10.81 (1679–18158) |
|
| G0272042 8.53 (459–3915) | ||
| Translation regulation |
|
Rpl32* 79.86 (7–559) |
| R52*a 10.44 (195–2035) | ||
| Other functions |
G0277795 -23.55 (259.11) |
G0284969* 12.87 (93–1197) |
| cog2* 12.41 (539–6687) | ||
| G0276325* 32.62 (8–261) | ||
| G0290965*c 12.31 (13–160) |
The following information is indicated in each line of columns 2 and 3: the reference number of the gene, the ratio of the number of reads in wild-type and mutant samples (negative when a larger number of reads was obtained for the mutant strain) and the actual number of reads for each sample (mutant-wild type). Asterisks on the third column indicate genes that present a sequence related to Mef2 binding sites in their putative promoter regions. The letter “c” indicates that this sequence correspond to the consensus Mef2 binding site. The letter “a” indicates the presence of consensus binding sites for the S. cerevisiae Arg80 MADS-box transcription factor.