Table 3.
Primers used for promoter CpG analysis
| Primer code | Primer sequence (5′-3′)* | Amplicon length (base pairs) | Number of CpG sites analyzed |
|---|---|---|---|
| VEGF_F1 |
aggaagagagTTAGAAATAGGGGGTTTAGGAGTAAAT |
465 |
23 |
| VEGF_R1 |
cagtaatacgactcactatagggagaaggctCCAAATTCACAACCTAAAAATTACCCA |
||
| FLT1_F1 |
aggaagagagGTTTTTAGGAAGTAGAAGATTGAGG |
444 |
30 |
| FLT1_R1 |
cagtaatacgactcactatagggagaaggctCTCAAAAAATCCTTACAAACTCCCT |
||
| KDR_F1 |
aggaagagagTTTTAGAGTGGGTTTTTTATTTATAGAGG |
403 | 37 |
| KDR_R1 | cagtaatacgactcactatagggagaaggctAACTCCCACCCTACACTAAATCC |
* T7-promoter sequence tag -cagtaatacgactcactatagggagaaggct added to reverse primer.
10-mer sequence tag- aggaagagag added to the forward primer.