Table 1.
Target | Primer | Primer Sequence (5′-3′) | Sequence Length |
---|---|---|---|
Luciferase |
Luciferase-S |
5′-TCgAAgCTTACCATggAAgACgCCA -3′ |
1677 bp |
Luciferase-AS |
5′-gAATggTACCTTACAATTTggAC -3′ |
||
Vimentin |
Vimentin-5UTR-AS |
5′ ATCggCTAgCgCgTCCCCgCgCCAg -3′ |
144 bp |
Vimentin-5UTR-AS |
5′-gAATAAgCTTggCTgCggAgggT -3′ |
||
Vimentin-3UTR-S |
5′-ATCgggTACCAAATTgCACACAC -3′ |
325 bp |
|
Vimentin-3UTR-AS |
5′-gAATgCggCCgCgAAgCAgAACC -3′ |
||
Myosin |
Myosin-5UTR-S |
5′-TCggCTAgCgAAggCTAAgCA -3′ |
154 bp |
Myosin-5UTR-AS |
5′-AATTAAgCTTACCTgAACCTg -3′ |
||
Myosin-3UTR-S |
5′-ATCgggTACCgCCTCTTCTCCTg -3′ |
1389 bp |
|
Myosin-3UTR-AS |
5′-gAATgCggCCgCgTgATgCTCAg -3′ |
||
MLV Packaging signal | psi-S |
5′ = AAAgTggTACCgggAggTAAgCT-3′ |
322 bp |
psi-AS | 5′-gCCTTggTACCgAACTgTTTTAg-3′ |
Sequence amplified for modifying the vectors (restriction sites used allowing the cloning are in bold).