Skip to main content
. 2013 Apr 16;13:35. doi: 10.1186/1472-6750-13-35

Table 1.

Primers for PCR amplification

Target Primer Primer Sequence (5′-3′) Sequence Length
Luciferase
Luciferase-S
5′-TCgAAgCTTACCATggAAgACgCCA -3′
1677 bp
Luciferase-AS
5′-gAATggTACCTTACAATTTggAC -3′
Vimentin
Vimentin-5UTR-AS
5′ ATCggCTAgCgCgTCCCCgCgCCAg -3′
144 bp
Vimentin-5UTR-AS
5′-gAATAAgCTTggCTgCggAgggT -3′
Vimentin-3UTR-S
5′-ATCgggTACCAAATTgCACACAC -3′
325 bp
Vimentin-3UTR-AS
5′-gAATgCggCCgCgAAgCAgAACC -3′
Myosin
Myosin-5UTR-S
5′-TCggCTAgCgAAggCTAAgCA -3′
154 bp
Myosin-5UTR-AS
5′-AATTAAgCTTACCTgAACCTg -3′
Myosin-3UTR-S
5′-ATCgggTACCgCCTCTTCTCCTg -3′
1389 bp
Myosin-3UTR-AS
5′-gAATgCggCCgCgTgATgCTCAg -3′
MLV Packaging signal psi-S
5′ = AAAgTggTACCgggAggTAAgCT-3′
322 bp
psi-AS 5′-gCCTTggTACCgAACTgTTTTAg-3′

Sequence amplified for modifying the vectors (restriction sites used allowing the cloning are in bold).