Table 3.
Strains and plasmids used in this study
Material | Relevant featuresa | Source |
---|---|---|
Strains |
|
|
Streptococcus infantarius subsp. infantarius | ||
CJ18 |
Wild type strain, suusac isolate |
[2,7,31] |
CJ18/pVE6007 |
CJ18 derivative carrying pVE6007, CmR |
this study |
CJ18ΔlacIIC |
lacIIC::pLFB1005, lacIIC gene disruption derivative of CJ18, EmR |
this study |
CJ18ΔlacZ |
lacZ::pLFB1006, lacZ gene disruption derivative of CJ18, EmR |
this study |
CJ18ΔlacS |
lacS::pLFB1007, lacS gene disruption derivative of CJ18, EmR |
this study |
Lactococcus lactis |
|
|
LL302 |
RepA+ derivative of MG1363, host for pORI28 |
[51] |
Plasmids |
|
|
pORI28 |
EmR, Ori+, RepA-, pWV01 derivative, vector for chromosomal insertions in Gram-positive bacteria |
[52] |
pVE6007 |
CmR, thermosensitive derivative of pWV01, carrier plasmid for pORI28 |
[53] |
pLFB1005 |
EmR, pORI28 derivative containing a 939-bp internal fragment of lacIIC. |
this study |
pLFB1006 |
EmR, pORI28 derivative containing an 1177-bp internal fragment of lacZ. |
this study |
pLFB1007 | EmR, pORI28 derivative containing a 900-bp internal fragment of lacS. | this study |
aCmR Chloramphenicol resistant; EmR Erythromycin resistant.