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. 2013 Mar 22;14:200. doi: 10.1186/1471-2164-14-200

Table 3.

Strains and plasmids used in this study

Material Relevant featuresa Source
Strains
 
Streptococcus infantarius subsp. infantarius
CJ18
Wild type strain, suusac isolate
[2,7,31]
CJ18/pVE6007
CJ18 derivative carrying pVE6007, CmR
this study
CJ18ΔlacIIC
lacIIC::pLFB1005, lacIIC gene disruption derivative of CJ18, EmR
this study
CJ18ΔlacZ
lacZ::pLFB1006, lacZ gene disruption derivative of CJ18, EmR
this study
CJ18ΔlacS
lacS::pLFB1007, lacS gene disruption derivative of CJ18, EmR
this study
Lactococcus lactis
 
LL302
RepA+ derivative of MG1363, host for pORI28
[51]
Plasmids
 
 
pORI28
EmR, Ori+, RepA-, pWV01 derivative, vector for chromosomal insertions in Gram-positive bacteria
[52]
pVE6007
CmR, thermosensitive derivative of pWV01, carrier plasmid for pORI28
[53]
pLFB1005
EmR, pORI28 derivative containing a 939-bp internal fragment of lacIIC.
this study
pLFB1006
EmR, pORI28 derivative containing an 1177-bp internal fragment of lacZ.
this study
pLFB1007 EmR, pORI28 derivative containing a 900-bp internal fragment of lacS. this study

aCmR Chloramphenicol resistant; EmR Erythromycin resistant.