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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Biochem J. 2010 May 13;428(2):255–267. doi: 10.1042/BJ20100090

Figure 4. Inhibition of PI3K partially blocks PDGF-induced changes in glycolytic flux.

Figure 4

ECAR values of VSMC treated with vehicle (CTRL) or PDGF (10 ng/ml) in the absence or presence of LY-294002 (LY; 10 μM). (A) Basal and oligomycin-stimulated ECAR values from cells treated for 24 h with vehicle or PDGF in the absence or presence of LY. The rates were normalized to total protein to give mpH units/min/μg protein. (B) Baseline ECAR group data for the treatment groups in panel A. (C) Group data of the oligomycin-dependent ECAR values for treatment groups in panel A. (D) Effects of acute PDGF exposure on glycolytic flux: VSMC were exposed to vehicle (CTRL) or PDGF in the absence or presence of LY for 5 min and ECAR rates were measured. (E) Effects of acute PDGF exposure on glycolytic flux after oligomycin addition. After acute administration of PDGF, cells were exposed to oligomycin (1 μg/ml) and ECAR rates were measured. Data are presented as means ± SEM; n=3–5 per group, *p<0.05 vs. CTRL, #p<0.05 vs. PDGF.