Fig. 6.

Effects of deletions in the gtaR promoter region on the binding of GtaR-6xHis. On the left are representations of the DNA fragments used in the EMSAs. The black boxes represent putative −10 and −35 sites, the empty box surrounding the left-most −10 site is the predicted lux box, and the grey bar indicates the protected region identified by DNase I footprinting. The size and name of each DNA fragment is given, and the corresponding EMSAs are on the right, with the concentration of the GtaR-6xHis protein in each reaction given above.