Comparison of RcGTA gene expression in quorum-sensing mutant strains of R. capsulatus.
A. β-Galactosidase activities of strains containing the orfg1::lacZ promoter fusion plasmid p601-g65.
B. Western blots of cells probed with RcGTA capsid protein antiserum.
C. Frequency of RcGTA-mediated gene transfer. The wild-type strain B10 is indicated by WT, mutant strains are indicated as ΔgtaI, ΔgtaR and ΔgtaRI, and the GtaR complement is indicated as ΔgtaRI(R); C16 indicates the ΔgtaI culture supplemented with C16 acyl-HSL. Error bars represent the standard deviation of the mean between samples (n = 3).