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. 2013 May 1;8(5):e61896. doi: 10.1371/journal.pone.0061896

Figure 3. Induction of Survivin by LR3-IGF-I in NRP-152 cell occurs through a PI3K/Akt/mTOR pathway.

Figure 3

(A) NRP-152 cells were infected with AdMax adenoviruses harboring WT-Akt, Myr-Akt (Act-Akt), AktK179M, KD-Akt, (p85αΔSH2N), CAAX-p110α (CA-PI3K), or treated with vehicle or LR3-IGF-I (2 nM) for 24 h. B) NRP-152 cells were infected with AdMax KD-PI3K overnight prior to a 24 h treatment with 2 nM LR3-IGF-I or vehicle. NRP-152 cells were pre-treated with 10 µM LY294002 (C), 5 µM perifosine (D), 1 µM MK2206, 200 pM rapamycin or 500 nM Ku-0036794 (E) overnight prior to a 24 h treatment with 2 nM LR3-PI3K or vehicle. Samples were subjected to Western blotting of Survivin. Results are representative of two to three independent experiments.