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. 2013 May 1;8(5):e62195. doi: 10.1371/journal.pone.0062195

Figure 4. PICT-FRAP assay.

Figure 4

(A) PICT-FRAP assay to analyze stable and transient interactions. A schematic representation of PICT-FRAP assay color-coded as in Figure 1. Ste11-FRB and Ede1-FRB were used as bait and Ste50-GFP and Syp1-GFP as prey in the respective experiments. For each assay, a frame from the GFP channel is shown corresponding to an anchoring site before, immediately after photobleaching and at the end of the measurements. (B) PICT-FRAP of the Ste11–Ste50 interaction (left) and the Ede1–Syp1 interaction (right). The curves represent the mean ± SD, Ste11–Ste50 (n = 8) and Ede1–Syp1 (n = 12).