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. 2013 May 1;8(5):e62331. doi: 10.1371/journal.pone.0062331

Figure 4. How close a second spot can be without affecting the calculated separation.

Figure 4

Simulations to determine how close, using distances in the range 3–8 pixels (as indicated to the left or right of each plot), a second spot can be before it affects the calculated separation within the first spot. If it is closer than 7 pixels (FWHM of the spot is 3 pixels), the separation is miscalculated and the percentage of consistent measurements does not reach 68%. Each plot shows data from 30 tracks. The simulated separation is 13 nm.