Figure 2. Evaluation of the capacity of F4/80⁺CD11b⁺, F4/80^lowCD11b⁺ and MHCII⁺CD11c^high populations to produce TNF-α and IL-12/IL-23p40 during the acute phase of T.cruzi infection.

Splenic cells were analyzed seven days post-infection. (A) Representative flow cytometry plots showing the exclusion of debris and non-interest population of interest (FSC-H X SSC-H) and the assortment of immune cells from non-infected or infected mice. Representative flow cytometry plots showing intracellular cytokine in the different cells from non-infected or infected mice. (B) Frequencies of IL-12/IL-23p40⁺ or TNF-α⁺ splenic cells (F4/80⁺CD11b⁺, F4/80^lowCD11b⁺ or MHCII⁺CD11c^high) (mean ± SD of four mice) isolated from non-infected or infected mice. Data are representative of two independent experiments. **p<0.01 indicates statistical significance when comparing the percentage of the same cell population from infected versus non infected mice involved in TNF-α or IL-12/IL-23p40 production.