Figure 2.

LPLUNC1 inhibits 5-8F NPC cell growth and proliferation. A. Western blot analysis of LPLUNC1 expression in 5-8F/vector and 5-8F/LPLUNC1 cells using the anti-GFP primary antibody revealed that 5-8F/LPLUNC1 cells expressed the GFP tagged LPLUNC1 protein, whereas 5-8F/vector cells expressed GFP protein alone. B. MTT assay of 5-8F/LPLUNC1 cells. A total of 1×10⁴ cells were seeded into 96-well, flat-bottom plates and cultured for 72 h, and 10 µl of MTT (5 mg/ml) was added. LPLUNC1 expression significantly inhibited cell proliferation; p<0.01. C. Growth curve of the LPLUNC1 stably transfected 5-8F cells. A total of 1×10⁴ cells were seeded into 24-well plates, and the number of cells were counted with a hemocytometer every 24 h. The expression of LPLUNC1 markedly inhibited cell growth; p<0.01. D. Soft agar colony formation assay of 5-8F/LPLUNC1 cells. Colonies were manually counted and imaged using microscopy and photographed two weeks later (magnification = 100×, Bar = 100 µm). E. The number of colonies per plate in the colony formation assay was derived from three independent experiments, with duplicates in each experiment. The data are presented as the mean ± SD; p<0.05. Both the size and number of the colonies formed in soft agar were decreased in 5-8F/LPLUNC1 cells compared with 5-8F/vector cells.