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. 2013 May 1;8(5):e62871. doi: 10.1371/journal.pone.0062871

Figure 4. DEP-1 regulates FL-stimulated FLT3 signaling. DEP-1 expression was stably downregulated in THP-1 cells by lentiviral transduction of shRNA.

Figure 4

Control cells harbor a non-targeting shRNA construct. (A) FL-dependent ERK1/2 activation was assessed by immunoblotting of cell lysate samples with antibodies recognizing activated ERK1/2 (pERK1/2). Loading was analyzed by reblot with ERK1/2 antibodies. Representative experiment; the efficiency of stable DEP-1 knockdown is also shown (upper panel). (B) Quantitative data for 4 independent experiments. Numbers represent pERK1/2 signals normalized to ERK1/2 levels in the same sample. The value of control cells at 10 min was set to 1.0. Significance for the difference between responses of the two different cell pools was determined by two-way ANOVA.