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. 2013 May 1;8(5):e63214. doi: 10.1371/journal.pone.0063214

Figure 4. mBSA elicits IL-1β production by activating inflammasome.

Figure 4

(A) Secretion of IL-1β by BMDM stimulated with different forms of BSA or Aβ peptides for 2 hours. Error bars are means ± SD of 5 independent experiments. ****p<0.0001. (B) Caspase 1 activation in BMDMs stimulated with different forms of BSA analyzed by FLICA assay. Representative result of 3 independent experiments is shown. Original magnification 100X. bar: 20 µm. (C) Acridine orange staining of BMDM stimulated by different forms of BSA to reveal lysosome integrity. Original magnification of fluorescence microscopy 100X. bar: 20 µm. (D) Secretion of IL-1β by BMDM induced by different forms of BSA in the presence of different inhibitors. Error bars are means ± SD of 2 independent experiments. *p<0.05, **p<0.005. (E) Secretion of IL-1β by BMDM induced by different forms of BSA or Aβ peptides in the presence or not of the NRLP3 inhibitor glybenclamide. Error bars are means ± SD of data obtained with cells from 4 different mice. *p<0.05, **p<0.005. (F) Secretion of IL-1β by BMDM induced by different forms of BSA in the presence of the caspase 1 inhibitor Ac-YVAD-CMK. Error bars are means ± SD of data obtained with cells from 3 different mice. *p<0.05, **p<0.005.