Figure 4. Reprogramming factors SOX2 and POU5F1 are PcG repressed and DNA methylated, respectively, in fibroblasts.

We compared PcG repression and DNA methylation in fibroblasts and an embryonic stem cell line (H1 ESC). (A) Of the six original reprogramming factors [31], [33] (labeled points), SOX2 is the most significantly PcG repressed in fibroblasts. Plot layout similar to Figure 1B. (B) In contrast, POU5F1 is the most differentially methylated reprogramming factor. Differential methylation is shown in units of absolute methylation score (ams). Data from [57], [58] and the Roadmap Epigenomics Project (Table S1).