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. 2013 May 1;8(5):e62012. doi: 10.1371/journal.pone.0062012

Figure 2. Treatment of human islets with MSDC-0160± IGF-1 prevents the loss of insulin content and maintains insulin secretion.

Figure 2

Islets were cultured in cCMRL, 5 or 8 mM glucose with or without the concentrations of MSDC-0160± IGF-1 for 4-days as indicated. (A) Islet insulin content was measured from 20 islets in duplicate at the start of the incubation period (time-0) and after 4-days of incubation under the conditions shown. Islets were sonicated in PBS with 0.05% BSA and kept at −20°C until assayed as described. (B) Islets were cultured in cCMRL with 8 mM glucose ± MSDC-0160. After 4 days, MSDC-0160 was removed and islets incubated for 90 min in cCMRL with 5 mM glucose. Insulin secretion was measured by a static incubation for 30 min with cCMRL containing 5 or 16.5 mM glucose ± forskolin. Triplicates of 10 islets were assayed. Insulin was measured by radioimmunoassay. Data are means±SEM from n = 3 (A) and n = 2 (B) with duplicate (A) or triplicate (B) samples in each experiment. Data was analyzed using one-way ANOVA followed by Newman-Keuls Post-hoc test (A) and two-way ANOVA followed by Bonferroni Post-hoc test (B).