Figure 8. Different CD8⁺ CTL populations were measured for interferon-γ (IFNγ) produce when stimulated with three breast cancer cells by ELISPOT assay.

1×10⁵ CD8⁺ CTLs were cultured in triplicate wells in 100 µl of complete medium containing 1×10³ breast cancer cells in 96-well anti-IFNγ antibody precoated plates. After 20 h incubation, IFNγ spots were analyzed. (A) displayed partly spots from a experiment; (B) The histogram shows the data of six independent experiments from two volunteers as mean ± SE. **indicates that this group has statistically significant differences compared to the others (p<0.01). * indicates that there are statistically significant differences compared to the groups without * (p<0.01).