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. Author manuscript; available in PMC: 2014 May 21.
Published in final edited form as: Chem Soc Rev. 2012 Dec 18;42(10):4345–4357. doi: 10.1039/c2cs35412h

Figure 3.

Figure 3

Enrichment strategies used to separate O-GlcNAc modified proteins from nuclear and cytosolic fragments. A) Use of O-GlcNAc specific antibodies and sWGA helps to bind a number of glycosylated proteins. B) Chemoenzymatic method using a mutant form of GalT that transfers a galactose analog bearing a chemically reactive group onto O-GlcNAc residues. Subsequent chemistry with an orthogonal probe allows for tagging and enrichment via biotin based labels