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Metabolic incorporation strategies and proteomic analyses of enriched O-GlcNAcylated samples. A) Metabolic incorporation of a variety of peracetylated analogs result in labeling of intracellular proteins with O-GlcNAc substrate analogs bearing a chemical handle. Bioorthogonal reactions assist in tagging of labeled proteins and their purifications. B) Tagged proteins analyzed via proteomic tools for peptide identification - ms/ms analysis and BEMAD technique for site determination.
