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. 2013 Apr 30;11(4):e1001550. doi: 10.1371/journal.pbio.1001550

Figure 11. JAGGED directly represses PETAL LOSS.

Figure 11

Expression levels (relative to the TUB4 constitutive control) of PTL mRNA measured by qRT-PCR in (A) wild-type and jag-1 inflorescence apices and (B) 35S::JAG:GR inflorescence apices 5 h after mock treatment, treatment with dexamethasone 10 µm (DEX), cycloheximide 10 µm (CHX), or cycloheximide 10 µm combined with dexamethasone 10 µm (CHX/DEX). (C) Chromatin immunoprecipitation (ChIP) using anti-GR antibodies and inflorescence apices of 35S::JAG:GR plants 4 h after mock treatment or treatment with DEX 10 µm. Target sequence 0.5 Kb upstream of the PTL start codon shows significant enrichment. Mu-like negative control did not show a significant difference (unpublished data). (A–C) Bars show the average and standard deviation of three biological replicates; asterisks indicate statistically significant differences (unpaired two-sample Student's t test, p<0.05) between the untreated wild type and jag-1 (A) and between DEX-treated samples and corresponding controls (B, C).