Figure 4.

Cga1 increases chloroplast development, A, Confocal microscopy of leaf blade from the Cga1 transgenics compared with wild-type Kaybonnet controls (Wt-Kay). B, Chloroplast number counted using a hemocytometer (n = 40+; *P < 0.05). C, Light microscopy showing increased chloroplast development in regions where they are not prevalent in wild-type plants. In the structural cells of the leaf midrib (top), the red arrows point to an immature etioplast in the wild type and a fully developed chloroplast in Cga1++. Epidermal cells of the leaf sheath (middle) sampled 1 cm from the base of the plant demonstrate increased chloroplast development in Cga1++. Root cells (bottom) also display aberrant chloroplast development in Cga1++ plants.