Table I. T1 progeny screening applied to 15 T0 families derived by self-pollination and two cross pollinations of wild-type cv M82 with T0 lines 7i and 7o.

All T0 plants harbored a nonmobile Ac element and a mobile Ds element. The progeny were screened for resistance to Liberty herbicide (BAR+) to identify those with the Ds element, followed by sensitivity to hygromycin leaf painting (hyg−) of herbicide-resistant seedlings to identify those with the Ac element. Screening efficiency measured the percentage of seedlings identified as putative transposants (herbicide resistant, hygromycin sensitive) that were verified as Ds only by multiplex PCR. Totals represent only a subset of T1 progeny screened during the project. For χ², expected was 3:1 BAR+/BAR− for T0 selfs, 1:1 for wild type × T0 crosses. **P < 0.01; ns, not significant, P > 0.05. Ds only (%) = (Ds only/total) × 100. Screening efficiency = (Ds only/hyg−) × 100. NA, Not applicable.

T0 Family	BAR+	BAR−	Total	BAR+:1 BAR−	χ2	hyg−	Ds Only	Ds Only	Independent Ds Only	Independent Ds Only	Screening Efficiency
								%		%
7a	498	474	972	1.1	292**	49	46	5	28	61	94
7b	609	187	796	3.3	0.9ns	144	68	9	42	62	47
7c	578	58	636	10.0	85**	141	102	16	34	33	72
7g	438	22	460	19.9	99**	91	81	18	39	48	88
7h	172	128	300	1.3	49**	0	0	NA	0	NA	NA
7i	804	443	1,247	1.8	73**	185	138	11	46	33	75
Wild-type × 7i	240	400	640	0.6	40**	52	42	7	25	60	81
7k	253	65	318	3.9	3.3ns	0	0	NA	0	NA	NA
7m	641	25	666	25.6	159**	134	90	14	22	24	67
7o	81	34	115	2.4	1ns	0	0	NA	0	NA	NA
Wild-type × 7o	267	293	560	0.9	1.1ns	0	0	NA	0	NA	NA
7p	310	21	331	14.8	60**	93	83	25	39	47	89
7r	264	105	369	2.5	2.2ns	59	40	11	18	45	68
7s	62	42	104	1.5	13**	26	18	17	6	33	69
7t	45	3	48	15.0	8**	12	11	23	7	64	92
7u	107	2	109	53.5	30**	30	27	25	12	44	90
7z	202	23	225	8.8	26**	38	33	15	3	9	87
Total	5,571	2,325	7,896			1,054	779		321		74