Figure 10.

Phenotypes of copt2-1 seedlings grown under Pi, Cu, and Fe deficiencies. A, Representative photographs of 7-d-old seedlings from the wild type (WT) and the copt2-1 line grown under Pi starvation (−Pi) and the conditions described in Figure 3A with regard to Cu deficiency (−Cu) and Cu and Fe deficiencies (−Cu−Fe). B, Root lengths from the wild type (white bars) and copt2 (gray bars) measured in three biological replicates ± sd in the samples described in A. Asterisks above the bars represent significant differences among all the means (P < 0.05) compared with the wild type. C, Overnight GUS staining in 7-d-old seedlings from the PCOPT2:GUS transgenic line grown under Pi deficiency (+Fe+Cu−Pi), Cu and Pi deficiency (+Fe−Cu−Pi), and Fe and Pi deficiency (−Fe+Cu−Pi). [See online article for color version of this figure.]