Figure 8.

Gene expression pattern of Cu deficiency marker genes in copt2-1 seedlings. Expression analysis is shown for the Cu transporter COPT1 (A) and the FeSOD and Cu/ZnSOD genes FSD1 (B) and CSD2 (C), respectively, by qPCR in wild-type (WT; white bars) and copt2-1 (gray bars) seedlings, grown under the same conditions described in Figure 3. UBQ10 gene expression was used as a loading control. Values are means ± sd of three biological replicates. r.u., Relative units. Different letters above the bars represent significant differences among all the means (P < 0.05).