Figure 11.

Epistasis analysis of ARS73a and SNRK2.2. A, Accumulation of proteins encoding the high-affinity SLT SO₄²⁻ transporters in the wild type (WT; 21gr), ars73a, snrk2.2, and snrk2.2 ars73a after 24 h of S starvation. A ferroxidase, FOX1, served as a loading control. B, SO₄²⁻ uptake in wild-type C. reinhardtii cells (21gr) and the ars73a, snrk2.2, and snrk2.2 ars73a mutant strains. Uptake rates were determined at 250 μm SO₄²⁻ (specific activity of 330 Ci mol⁻¹). The values are averages of a single experiment with three technical replicates, although similar results were obtained in independent experiments, and the error bars represent 1 sd.