Figure 7.

ARS73a mRNA in 21gr (wild type), ars73a, ars73a-BC (backcrossed ars73a), and the rescued strain (2-3A). The cells were grown under S-replete conditions or exposed to 6 h of S-depleted conditions prior to RNA isolation. First-strand cDNA was amplified with primers flanking the insertion in intron 6 (amplifying exons 4–7). The expected product derived from the wild-type transcript is 596 bp. A longer 733-bp product is observed in the ars73a mutant and the backcrossed mutant under S deprivation conditions. The CBLP transcript was used as a loading control. The sequences of these two mRNAs are given in Supplemental Figure S5.