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RT-qPCR analysis of transcript accumulation in sac1, snrk2.1, and the double mutants sac1 ars73a and snrk2.1 ars73a. Transcripts encoding SULTR2, SLT1, SLT2, AOT4, SULTR1, ARS1, ARS2, ECP76, SBDP, LHCBM9, and HAP2 were quantified by RT-qPCR. RNA samples were isolated from cells grown in S-replete medium (white bars) and at 4 h (gray bars) and 24 h (black bars) following the transfer of cells to medium devoid of S.
