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Yeast one-hybrid analysis of selected WRKY transcription factors against W-boxes of AOX1a, NDB2, and BCS1 promoters and a negative control (promoter region without the W-box). Non-WRKY transcription factor ABF4 was also included as a negative control for bait self-activation. Three 10-fold dilutions were spotted vertically for each assay on DDO (transformation control) and TDO (interaction) media, including p53 positive and negative controls.
