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Degradation of YFP-IAA fusion proteins in yeast in the presence of wild-type and mutant TIR1 proteins after the addition of auxin. Yeast cells were imaged using time-lapse flow cytometry. Degradation curves were normalized to the starting fluorescence. a.u., Absorbance units; D, Asp; D/M, Asp/Met; M, Met; WT, wild type.
