Figure 6.

ProTIR1:tir1 D170E/M473L plants exhibit growth defects. A, After 10 d of growth, ProTIR1:tir1 D170E/M473L-Myc 6-10 seedlings have more lateral roots than the wild type. B, Four-day-old seedlings were transferred onto medium with and without 2,4-D for an additional 6 d. The number of emerged lateral roots per mm of primary root length was determined. C, ProTIR1:tir1 D170E/M473L-Myc plants have smaller rosettes compared with the control line. Plants are 3 weeks old. D and E, ProTIR1:tir1 D170E/M473L 6-10 plants have fewer axillary branches compared with the control. WT, Wild type. Bar = 1 cm. Plants are 7 weeks old. For both B and E, error bars are se. Asterisks denote statistically significant differences between the tir1 D170E/M473L-Myc and TIR1-Myc control plants (Student’s t test, P < 0.05).