Figure 7.

The ProTIR:tir1D170E/M473L lines are auxin hypersensitive. A, Root growth on increasing concentrations of 2,4-D relative to untreated controls. B, Expression of auxin-responsive genes in ProTIR1:tir1D170E/M473L-Myc plants. Seven-day-old seedlings were analyzed by quantitative PCR. Data shown are from three biological replicates. Error bars are se. Asterisks denote statistically significant differences between the tir1 D170E/M473L-Myc and TIR1-Myc control plants (Student’s t test, P < 0.05).