Quantification of calcium, other minerals, and micronutrients in red ripe stage fruit from transgenic line E8.2-OXDC-11. Levels of calcium (A), magnesium (B), and micronutrients iron (C), manganese (D), and zinc (E) were determined using atomic absorption spectrophotometry. Data represent the values obtained from the means (± sd) of four plants, two fruits per plant for each independent transgenic event and the wild type in triplicate represented by error bars. Asterisks indicate significant difference (P < 0.05) analyzed by Wilcoxon rank sum test.