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. 2013 Mar 29;162(1):39–51. doi: 10.1104/pp.113.216994

Figure 7.

Figure 7.

Subcellular localization of pPLAIIIδ in Arabidopsis. A, Confocal imaging of epidermal cells of wild-type leaf (a–d) and pPLAIIIδ-OE:GFP leaf (e–g) chlorophyll fluorescence (a and e; red) versus GFP (b and f; green). The green fluorescent signal of the GFP-tagged pPLAIIIδ protein is shown in f. Transmitted light (c) and overlay (d) clarify cell outlines. Bar = 50 µm. B, Plasmolysis of root epidermal cells of the pPLAIIIδ-OE:GFP mutant: a, at 1 min after plasmolysis, the green fluorescence signal was located close to the cell wall; b and c, at 3 and 5 min after plasmolysis, respectively, the green fluorescence signal was colocalized with plasma membrane during cell shrink. Bar = 50 µm. C, Immunoblotting of pPLAIIIδ-GFP using GFP antibodies in subcellular fractions. Soluble protein (20 µg per lane) and membrane protein (5 µg per lane) were used in SDS-PAGE, followed by immunoblotting. Cytosol, Soluble fraction; MM, microsomal membrane fraction; PM, plasma membrane; IM, intracellular membrane.