Seed-specific OE of pPLAIIIδ increases oil content without compromising seed production. A, Plant heights of mature wild-type (WT), pPLAIIIδ-KO, 35S::pPLAIIIδ, and CON::pPLAIIIδ plants. 35S represents the cauliflower mosaic virus 35S promoter, and CON represents the promoter of soybean β-conglycinin. Values are means ± se (n = 5). B, Seed yield per plant of wild-type, pPLAIIIδ-KO, 35S::pPLAIIIδ, and CON::pPLAIIIδ plants. Values are means ± se (n = 5). C, Transcript levels of pPLAIIIδ in developing siliques of the wild type and three independent CON::pPLAIIIδ lines. The RNA level was determined by real-time PCR and normalized in comparison with UBQ10. Values are means ± se (n = 3). D, Seed oil contents in the wild type and three independent CON::pPLAIIIδ lines. Values are means ± se (n = 3). E, Fatty acid (FA) composition in wild-type and CON::pPLAIIIδ seeds. Values are means ± se (n = 3). HSignificantly higher and Lsignificantly lower, each at P < 0.05 compared with the wild type, based on Student’s t test.