Figure 2.
Molecular analysis of ZFN-mediated mutagenesis events in tobacco (T23, T8-21, T10-3, and T23-6) and Arabidopsis (A17-5 and A17-6) plants. The QQR ZFN-binding sites, on the top strand of the parent target sequences, are in blue, and nucleotide changes to the parent target sequences are in red. gfp, GFP coding sequence; P, CaMV dual 35S promoter; T, CaMV 35S terminator. Dashes indicate base deletions. [See online article for color version of this figure.]