Figure 3.
NHEJ-mediated gene deletion in plants. A, Structure of the target gene region and outcome of gfp deletion events. The locations and names of primers used for PCR amplification of the targeting events are shown. gfp, GFP-encoding gene; P1, CaMV dual 35S promoter; QQR, QQR ZFN recognition site; QQR*, altered QQR ZFN recognition site; T1, CaMV 35S terminator. B, PCR analysis of wild-type (WT), parental (PR), and gfp-deleted (T8) tobacco genomes. M, DNA ladder. C, Confocal microscopy analysis of leaf tissue from parent transgenic (PR) and gfp-deleted targeted (T8) plants. GFP expression is in green, and plastid autofluorescence is in red. Images are single confocal sections. Bars = 40 μm. [See online article for color version of this figure.]