Figure 7.

Phenotypical analyses and sexual transmission of replacement events. A, Phenotypical analysis of a gfp replacement event in Arabidopsis. The wild type (WT), a parental line (PR), and offspring of targeted line A15 were grown on germination medium supplemented with kanamycin and with (+H) or without (−H) hygromycin. B, Phenotypical analysis of a gfp replacement event in tobacco. The wild type, a parental line, and offspring of targeted line T19 were grown on germination medium supplemented with kanamycin and with or without hygromycin. C, Shoot formation from leaf discs of targeted tobacco line T14 (with or without inoculation with p19-encoding T-DNA) on hygromycin-containing selection medium. D, Shoot formation from leaf discs of a parental tobacco line (with or without inoculation with p19-encoding T-DNA) on hygromycin-containing selection medium. [See online article for color version of this figure.]