Figure 2.

Light regulation of transcription and histone acetylation on six C4 genes in maize. A, Relative quantification of hnRNA expression levels of the six C4 genes in leaves from plants that were exposed to 72 h of darkness (72D; gray columns) and in plants that were illuminated for 4 h (4L; black columns). Transcription is standardized for maize Actin1 expression (relative enrichment [RE]). hnRNA expression levels were determined by quantitative reverse transcription (RT)-PCR with a primer system specific for an intron (Supplemental Fig. S1). B, Light-dependent H3K9ac, H4K5ac, and H3K18ac on positions Zm1 and Zm2 of the six C4 genes. Values are presented as the relative enrichment of modifications per nucleosome over modifications per nucleosome found on the Actin1 promoter. All data points are based on at least four independent experiments. Vertical lines indicate se.