Figure 4.

Suppression of F3H disrupted petal color and fiber development. Flowers at 1 DPA (A), total anthocyanins of the petals (B), and real-time PCR (C), northern blotting (18S RNA as a reference; D), and enzyme assay (E) of F3H in 5-DPA ovules were analyzed to characterize the transgenic plants. Analysis of homozygous T2 mature plant fibers showed that the fiber length (F and G) and micronaire (H) were significantly decreased in F3H-RNAi plants. Error bars represent the sd of three biological replicates. Asterisks in B, G, and H indicate significant differences by Student’s t test (P < 0.05). Ri, RNAi plants; CK, the wild-type plant; OX, overexpression plants.