Fig. 2.
Simulated light scattering (LS) signals for: a single-species concentration gradients (solid blue line no interaction, dotted green line monomer–dimer KD = 10 μM, dashed red line monomer–trimer KD = 10 μM, dash–dot black line monomer–dimer KD = 1 μM), b crossover gradient of Fig. 1b assuming 1:1 binding at three different affinities (solid blue line no interaction, dotted green line KD = 100 nM, dashed red line KD = 10 nM, dash–dot black line KD = 1 nM), c crossover gradient of Fig. 1b assuming different stoichiometries (protein X:protein Y) at constant per-site binding affinity [solid blue line no interaction, dotted green line 1:1, dashed purple line 2:1, dash–dot red line 3:1, long-dash-gray line 2:2 (KD for 2:2 is 10× that of the other stoichiometries)]