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. 2013 Apr 12;3(4):e109. doi: 10.1038/bcj.2013.6

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Copyright © 2013 Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

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LY2784544 selectively inhibits STAT5 phosphorylation in Ba/F3 cells expressing TEL-JAK2 than Ba/F3 cells expressing TEL-JAK1 and JAK3. Ba/F3 cells expressing TEL-JAK1, TEL-JAK2 and TEL-JAK3 were treated with the indicated concentrations of LY2784544, ruxolitinib or DMSO (0, vehicle control) for 2 h and cell lysates were probed for pSTAT5^y694 and total STAT5 by western blotting. Ba/F3, murine pro-B-cells; DMSO, dimethylsulfoxide; JAK, janus kinase; STAT5, signal transducer and activator of transcription 5; TEL, translocated ets leukemia.