Figure 2.

Nuclear BNIP3 suppresses the expression of DR5 at the RNA level. (a) U251 cells were transiently transfected with GFP (control), wild-type BNIP3, shRNA against BNIP3 and BNIP3 targeted to the nucleus (NLS). Fold changes in the expression of DR5 mRNA were determined using the ΔΔC_T method and all fold changes were normalized to the GFP transfection control. Human cyclophilin was used as the control gene for the experiment. Error bars represent the S.E. determined from three independent experiments. * denotes a P-value <0.05 representing statistical significance between wild-type BNIP3-transfected cells, shRNA and BNIP3-NLS-transfected cells compared with GFP-transfected cells. (b) Total RNA was isolated from the brains of BNIP3 wild-type or knockout mice. Fold changes in the expression of DR5 mRNA were determined using the ΔΔC_T method and all fold changes were normalized to the wild-type mouse. Mouse 18s was used as the control gene for the experiment. Error bars represent the S.E. determined from three independent experiments. * denotes a P-value <0.001 representing statistical significance between wild-type BNIP3 mice and BNIP3 knockout mice