Figure 6.

TTC5 and EP300 promote MYC turnover. THP1 cells were infected with lentiviruses expressing shRNAs targeting TTC5 or EP300 for KD, or a non-targeting control shRNA (NTC), with puromycin drug resistance as the selectable marker. Bar charts show (a) mean±S.E.M. mRNA levels of MYC and TTC5 in TTC5 KD cells and (b) mean±S.E.M. mRNA levels of MYC and EP300 in EP300 KD cells relative to control cells, 72 h after lentiviral infection (n=3). Representative western blots show (c) expression of MYC after EP300 or TTC5 KD (n=3) and (d) MYC half-life, as determined by incubation of control or KD cells with 10 μg/ml cycloheximide for the indicated times, 72 h after lentiviral infection (n=2). Vertical dashed line demarcates separate gels, although exposure time was identical. (e) MYC half-life was semiquantified by densitometric analysis using ImageJ software