Figure 2.

Aberrant spindle formation and cell cycle arrest induced by knockdown of centrosomal proteins. (a) The levels of centrosomal proteins in four cancer cell lines transfected with control siRNA (GL) or the indicated siRNAs targeting centrosomal proteins (C) were analyzed by western blotting 48 h after siRNA transfection. (b) HeLa cells 48 h after siRNA transfection were stained with PI, and their nuclear DNA content was analyzed by flow cytometry. Percentages of cells in sub G1, G1, S, and G2/M phases are shown. Data shown were obtained from representative experiments, which were repeated three times with similar results. (c) HeLa cells 48 h after siRNA transfection were stained with anti-α-tubulin antibody and DAPI. Bar, 20 μm. (d) Percentages of HeLa cells harboring aberrant spindles were counted in more than 100 randomly selected mitotic cells 48 h after siRNA transfection