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. 2013 Apr 18;4(4):e603. doi: 10.1038/cddis.2013.108

Figure 5.

Figure 5

Apoptosis following mitotic catastrophe induced by knockdown of centrosomal proteins depends on the SAC. (a) The levels of Bub1, BubR1, and Mad2 proteins in HeLa cells transfected with the corresponding siRNAs were analyzed by western blotting 48 h after siRNA transfection. (b) siRNAs against SAC factors and centrosomal proteins were transfected simultaneously. Cell images were acquired using a phase-contrast microscope 48 h after siRNA transfection. Bar, 50 μm. (c) Percentages of arrested cells at M phase following cotransfection with siRNAs against centrosomal and SAC proteins 48 h after siRNA transfection. Over 200 randomly selected cells were counted for each sample. Values are an average of three independent experiments. Error bars represent S.D. (d) Rates of cell death (expressed as percentages) induced by knockdown of centrosomal and SAC proteins 48 h after siRNA transfection. Over 200 randomly selected cells were counted for each sample. Values are an average of three independent experiments. Error bars represent S.D.